GC-MS STUDY OF IXORA PAVETTA VAHL.HTML Full Text
GC-MS STUDY OF IXORA PAVETTA VAHL.
- Srinivas 1 and R. V. Celestin Baboo*2
Sri Vasavi Institute of Pharmaceutical Sciences 1, Pedatadepalli, Tadepalligudem, West Godavari Dt., Andhra Pradesh, India
- M. Reddy Memorial College of Pharmacy 2, Petlurivaripalem, Narasaraopet, Guntur Dt., Andhra Pradesh, India
Ixora pavetta Vahl. is a small tree, traditionally used for the treatment of diarrhoea, dysentery, urinary disorders, leucorrhoea, veneral diseases and sedative. The flowers of the plant were extracted with ethanol by cold percolation method. The presence of the compounds in the extract was analyzed by GC-MS technique. The parameters of mass spectral analysis viz. retention time and characteristics of peak using NIST library were considered for the detection of compounds, The compounds viz. 3-Butyn-2-ol, 3-Butyn-1-ol, Amyl nitrite, 2Octyn-1-ol, 1, 9-Decadiyne and Butyl glyoxylate were identified from the study. With reference to the phytochemical and ethanobotanical databases, the Pharmacological activity of the compounds which identified were found out and compared with its traditional uses. Sedative property is one of the traditional uses of Ixora pavetta was found to be due to the presence of Amyl nitrite in the extract.
INTRODUCTION: Ixora pavetta is a small tree or evergreen shrub belongs to the family Rubiaceae found in deciduous slopes and hills. The leaves of the plant are elliptic-oblong to oblanceolate, entire margin, obtuse-acute apex. The flowers are white, calyx truncate & four toothed, corolla tubular, lobes four, obtuse, satmens four, anthers basally tailed, and drupes didymous. Traditionally the plant is used for the treatment of diarrhoea, dysentery, urinary disorders, leucorrhoea, veneral diseases and sedative 1, 2.
The object of the present study was to detect the causative matters for its traditional uses by GC-MS analysis, phytochemical and ethno botanical databases. Gas chromatography Mass spectroscopy (GC-MS) technique is one of the excellent methods to detect the presence of compounds in the crude extracts. The parameters viz. retention time, base peak area, characteristics of peak using National Institute of Standards &Technology (NIST) Library were considered for the detection of compounds in GC-MS analysis. Phytochemical and ethnobotanical databases provide the information regarding the pharmacological activity of the compounds. The flowers of the plant are aimed to extract with alcohol by cold percolation method and detect the presence of compounds with the help of GC-MS analysis. Also planned to find out the pharmacological activities of the compounds which is identified and compare with its traditional uses.
MATERIALS AND METHODS:
Flowers: Attractive, white colored, agreeable and aromatic odorous flowers of Ixora pavetta were collected from Nagercoil, Tamil Nadu, during February 2010. The collected flowers were authenticated by Research officer, V. Chellathurai, Department of Botany, Tirunelveli, Tamil Nadu.
Preparation of the extract: The authenticated flowers were dried under shade by spreading as a thin layer and ground to coarse powder. The powdered material was extracted by cold percolation method using ethyl alcohol as solvent. The percolation was carried by conical metal percolator. Glass wool moistened with alcohol and was placed at the bottom of the percolator. Alcohol moistened powder was added into the percolator and packed uniformly in order to facilitate free flow of menstrum through the drug. A piece of filter paper was placed on the packed drug.
Washed sand was added on the filter paper. A sufficient quantity of alcohol was added to saturate the material. The percolator was closed to prevent evaporation of alcohol. After 24 hours of maceration the outlet was opened and collected the percolates 3, 4. The percolation was continued till the drug was completely exhausted. The combined percolates were dried under vacuum dryer .The extract obtained was reddish brown in color, hard and brittle in nature. It yields about 7.2 % w/w of ethanol extractive value.
GC-MS Study: Ethanolic extract of flowers of Ixora pavetta was analyzed for the presence of different compounds by Gas chromatography-Mass spectroscopy (GC-MS) technique. It was carried out in GC Clarus 500 Perkin Elmer equipment with Turbo mass 5.2 Software. The column was packed with 5% Dimethyl and 95% Dimethyl poly siloxane. The sample of extract was dissolved in ethanol and 2µl of the same was injected for the determination. It was run for about 36 minutes and detection was carried by Mass detector Turbo mass gold-perkin Elmer. The method followed in the instrumentation of GC-MS analysis is mentioned in Table 1.
RESULTS AND DISCUSSION: The presence of six compounds in the ethanolic extract of flowers of Ixora pavetta by GC-MS analysis has shown in Table 2 and Fig. 1. Mass spectroscopy analysis using National Institute of Standards and Technology (NIST) Library and retention time programme confirmed the presence of these six compounds. The result showed that 3-Butyn-2-ol, 3-Butyn-1-ol, Amyl nitrite, 2-Octyn-1-ol, 1, 9-Decadiyne and Butyl glyoxylate were present in the extract. The pharmacological activities of the identified compounds were shown in Table 3.
TABLE 1: METHOD OF INSTRUMENTATION (GC-MS)
|Gas chromatography Programme|
|Equipment||GC Clarus 500 Perkin Elmer|
|Detector||Mass detector Turbo mass gold-Perkin Elmer|
|Column||Elite (5% Diphenyl/95% Dimethyl polysiloxane) 30 x 0.25mm x 0.25µm df|
|Carrier gas||1ml/minute, Split: 10:1|
|Total GC Running time||36 minutes|
|Mass spectroscopy Programme|
|Library used||NIST Version-Year 2005|
|Inlet line temperature||200o C|
|Source temperature||200o C|
|Electron energy||70 Ev|
|Mass scan ( m/z)||45-450|
|Solvent Delay||0-2 min|
TABLE 2: GC-MS ANALYSIS OF ETHANOLIC EXTRACT OF IXORA PAVETTA
|Name of the Compound
FIG. 1: GC-MS ANALYSIS OF ETHANOLIC EXTRACT OF FLOWERS OF IXORA PAVETTA
TABLE 3: THE COMPOUNDS IDENTIFIED FROM THE EXTRACT AND ITS ACTIVITIES
|Name of the compound||Nature of the Compound||Activities of the compound|
|Amyl nitrite||Nitrite||Angina pectoris, cyanide poisoning, Sedative|
|Butyl glyoxylate||Oxylate||Not found|
CONCLUSION: Ixora pavetta plant is traditionally used for diarrhoea, dysentery, urinary disorders, leucorrhoea, veneral diseases and sedative. The compounds identified by the GC-MS study from the same plant also have the similar properties. From this study, we have concluded that the causative matter for its traditional properties has been confirmed by the scientific approach.
- Madhava Chetty K., Sivaji K.and Tulasi Rao K.,Flowering Plants of Chittoor District of Andhra Pradesh, 2008, 158.
- Matthew, K. M. The flora of the Tamilnadu Carnatic. (F TamilC), 1983, 67.
- Rawlins E.A.,Bentley,s Text book of Pharmaceutics, E.L.B.S., 1982, 8th edition, 174-176.
- Mehta R.M. Pharmaceutics-I, Reprint 2004, 152-155.
K. Srinivas and R. V. Celestin Baboo*
Asst. Professor, Department of Pharmacognosy, A.M. Reddy Memorial College of Pharmacy, Petlurivaripalem Post, Narasaraopet (Mdl), Guntur District, Andhra Pradesh, India
22 April, 2011
02 June, 2011
11 July, 2011
01 August, 2011