GREEN SYNTHESIS OF NANOPARTICLES AND THEIR ANTIBACTERIAL ACTIVITY AGAINST PATHOGENIC BACTERIAAbstract
The present study investigated the phytochemical analysis of the major bioactive constituents of medicinal important plant of Punica granatum peels in its aqueous extract and green synthesis of silver nanoparticles and their evaluation for antibacterial activity. Flavonoid, phenol & tannins, carbohydrates, glycosides and etc were highly concentrated in the peels extract. The synthesis and characterization of silver nanoparticles was confirmed by UV-Visible spectroscopy. UV-Visible spectroscopy of the reaction medium containing silver nanoparticles show the maximum absorbance peaks 430nm (1%), 373nm (3%), 379nm (5%). Green synthesis of silver nanoparticles analyzed against bacterial cultures by using the disc diffusion method. The silver nanoparticles have shown bactericidal effects against E.coli (MTCC-40), Staphylococcus aureus (MTCC-7443), Proteus vulgaris (MTCC-*1771). The peels extract of Punica granatum quikely reduces Ag+ to Ago and enhance synthesis of silver nanoparticles with highly antibacterial activity.
Vijay Laxmee Mishra and Richa Sharma
Department of Biotechnology and Allied Sciences, Jayoti Vidyapeeth Women’s University, Jharna, Mahla – Jobner Link Road, Jaipur- Ajmer Express Way, Jaipur -303122, Rajasthan, India.
18 June, 2014
28 August, 2014
17 October, 2014
01 February, 2015