GREEN SYNTHESIS OF SILVER NANOPARTICLES USING ROOT EXTRACT OF AERVA TOMENTOSA FORSK. AND ANALYSE ANTIBACTERIAL AND ANTIOXIDANT PROPERTY

During the present investigations attempt was made to the green synthesis of silver nanoparticles using root extract of Aerva tomentosa Forsk. and to analyze its antibacterial and antioxidant activities. Exposure of the root extract of Aerva tomentosa to aqueous AgNO₃ resulted in the formation of silver nanoparticles. The synthesis of silver nanoparticles was confirmed by UV-Vis spectrophotometer and was characterized by X-ray diffractometer. The bactericidal activity of silver nanoparticles against Escherichia coli, Bacillus thuringiensis, Bacillus subtilis and Pseudomonas putida was determined using bacterial growth inhibition method. The antioxidant study of silver nanopartcles was analyzed by the 2, 2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging method. The synthesis of AgNPs via this green approach by Aerva tomentosa root extract were average size particles and crystalline in nature. The synthesized silver nanoparticles with two concentrations (0.01g/100µl) and (0.005g/100µl) exhibited high antibacterial activity in terms of maximum zone of inhibition against Escherichia coli, Bacillus thuringiensis, Bacillus subtilis, and Pseudomonas putida. Silver nanoparticles also showed significant antioxidant activity. There was a dose-dependent increase in the percent antioxidant activity for all concentrations (80, 160, 240, and 320 μg/ml of AgNPs) tested. Owing to its good antibacterial and antioxidant activity, present study supports that AgNPs might be used as potential antibacterial and antioxidant drug in traditional medicine to treat various diseases.