HELICOBACTER PYLORI INFECTION: A BIOINFORMATIC APPROACHAbstract
Helicobacter pylori causative agent of acid peptic disease is a microaerophilic, spiral-shaped, gram-negative bacteria found in the gastric epithelium that may also lead to complications such as chronic gastritis, mucosa-associated lymphoid tissue (MALT) lymphoma, gastroduodenal ulcer and adenocarcinoma in stomach. Plantbioactives have always been potential therapeutics. Usage of modern bioinformatic tools plays a vital role in exploiting the potentials of alternative therapeutic molecules in managing diseases like peptic ulcers and their complications. The in-silico evaluation was carried out using molecular docking of the quorum sensing proteins of H. pylori with the ligand β-sitosterol obtained from the silver nanoparticles of Acorus calamus L. Among several given quorum sensing proteins the molecular interaction with the ligand β-sitosterol showed a high binding affinity with DnaA, PhnB, ToxB and Sip proteins. The results obtained from molecular interaction study revealed that the ligand β-sitosterol will be readily taken up by the organism, thereby facilitating easy inhibition or inactivation of quorum sensing molecules ToxB, DnaA, PhnB, and Sip, making it a novel therapeutic alternative to treat H. pylori infections.
A. Prasad, G. Shruthi, P. Sushma, A. S. Jain, D. Chandan, M. N. N. Prasad, S. P. Kollur, C. Srinivasa and C. Shivamallu *
Division of Biotechnology and Bioinformatics, Department of Water & Health, Faculty of Life Sciences, JSS Academy of Higher Education & Research, Sri Shivarathreeshwara Nagara, Mysore, Karnataka, India.
12 February 2020
29 March 2020
31 March 2020
01 November 2020