HEPATOPROTECTIVE ACTIVITY OF A STANDARDIZED POLY- HERBAL LIVER FORMULATIONAbstract
The present study compiled the formulation and standardization of polyherbal liver syrup named “LivPro” followed by in-vivo and in-vitro hepatoprotective activity in CCl4 intoxicated rat. LivPro was formulated by using the lyophilized ethanolic extract of A. millefolium, C. spinosa, C. intybus, and P. kurroa. Further, the standardization of LivPro was performed following the guidelines of WHO and Indian Pharmacopoeia. Wistar albino rats were treated with vehicle, silymarin, and LivPro (5, 10 and 15 ml/kg) continuously for 14 days. CCl4 was administered on every alternate day for a week. Results postulated that LivPro had pH 5.04, specific gravity 0.8269; viscosity 78.01 poise, relative density 1.271 gm/ml, and refractive index 1.432. LivPro showed a rich quantity of flavonoid (480 mg/gm), total phenol (396 mg/gm) and alkaloid (103 mg/gm). The concentration of lead and arsenic in the tested formulation was found to be within the limit. The total plate count and viable aerobic microbial count in the formulation were 480, and 415 CFU/gm and the yeast and mold count was less than 100 CFU/gm. Pre and post-treatment of LivPro showed extremely significant (P<0.001) decrease in thiopental induced sleeping and 76.19% hepatoprotection in bromsulphthalein uptake test. Treatment with LivPro (10 and 15 ml/kg) resulted in significant normalization (P<0.01 to 0.001) of biochemical parameters except albumin. LivPro showed significant elevation (P<0.001) of glutathione and reduction (P<0.001) in lipid peroxidase enzymes in liver tissue. LivPro (15 ml/kg) treatment showed complete recovery of hepatocytes with reduced vaculation, clear cell lining as well as the absence of multinucleated giant cell.