HEPATOPROTECTIVE ACTIVITY OF HYDROALCOHOLIC EXTRACT OF BOERHAAVIA DIFFUSA LINN. AGAINST D-GALACTOSAMINE INDUCED HEPATOTOXICITY IN MICEAbstract
Boerhaavia diffusa is a natural herb possesses potent diuretic, anticancer and antioxidation properties. This work was designed to investigate the effect of hydro alcoholic extract of Boerhaavia diffusa Linn. on hepatoprotective efficacy in D-galactosamine-intoxication in mice. Albino Mice divided into 5 groups each consisting of 6 animals. Group 1 served as vehicle control, Group 2 served as hepatotoxin (D-Galactosamine treated) group, Group 3 served as positive control (Vitamin-E) treated group, Group 4, 5 served as treatment control (200 and 400 mg/kg b.w p.o.) HAEBD. Treatment given for 8 days and D-Gal on last day for all the groups except normal control. D-galactosamine-induced toxicity was manifested by the elevation of serum hepatic marker enzyme activities (Aspartate amino transferase, Alanine amino transferase, alkaline phosphatase and gamma-glutamyl transpeptidase). It also caused significant lipid peroxidation and reduced the levels of antioxidant defense mechanisms. Treatment with HAEBD (200 and 400 mg/kg body weight) orally for 8 days decreased hepatic marker enzyme activities and lipid peroxidation products, lipid hydroperoxides and conjugated dienes, increased the activities of free-radical scavenging enzymes superoxide dismutase, catalase and glutathione peroxidase and the levels of non-enzymatic antioxidants reduced glutathione, Vitamin C and Vitamin E. These findings demonstrate that HAEBD acts as a hepatoprotective and antioxidant agent against D-Galactosamine induced hepatotoxicity.
G. Nalini *, Ajithadasaruna, N. Chidambaranathan and N. Jegan
Department of Pharmacology, K. M. College of Pharmacy, Uthangudi, Madurai, Tamil Nadu, India.
01 December, 2017
21 February, 2018
04 March, 2018
01 August, 2018