HISTOARCHITECTURAL AND BIOCHEMICAL EVALUATION OF ISOPROTERENOL-INDUCED MYOCARDIAL ISCHEMIA IN HEART & LUNGS – AN EXPERIMENTAL DOSE-DEPENDENT STUDYAbstract
Background: Numerous methods have been used to induce myocardial ischemia (MI) in Experimental animals. MI induction using chemicals like isoproterenol in animal models is a simple and standardized method with fewer complications. Various doses of Isoproterenol were used extensively as an inducing agent. Aim: The present research aimed to evaluate the effect of Isoproterenol on the heart and lungs of male Wistar albino rats at different doses. Methods: Wistar albino rats were categorized as five groups with six animals each and administered with ISO for two days. Serum and histopathological studies were done. Results: Biochemical alterations and Microscopic features of the tissues showed pathological changes such as focal areas of inflammation, edema and vacuolar changes, and necrosis according to the dose administered, confirming myocardial injury and related necrotic lesions. Conclusion: These findings provide an idea and support fixing the dose for the inducing agent ISO for further cardioprotective studies.
Subbulakshmi Packirisamy *, Valli Gunam, Jaideep Mahendra, Parimala Kathirvelu and Deepa Rajendiran
Department of Pharmacology, Meenakshi Ammal Dental College, Meenakshi Academy of Higher Education & Research, Chennai, Tamil Nadu, India.
17 August 2022
11 April 2023
17 April 2023
01 May 2023