HISTOPATHOLOGICAL AND ULTRASTRUCTURAL STUDIES OF THE EFFECT OF FENUGREEK SEED EXTRACT ON PANCREAS OF ALLOXAN INDUCED DIABETIC MICEAbstract
To evaluate the protective effect of fenugreek (Trigonella foenum graecum L.) on alloxan induced diabetic mice, 15 male albino mice weighing 26-30 gm were used. They were divided into 3 groups. Control group received subcutaneous injection of 0.15 M acetate buffer for 15 days. Alloxan induced group received subcutaneous injection of alloxan 150 mg/ kg body weight to induce diabetes. Recovery group received subcutaneous injection of fenugreek seed extract 15 mg/kg body weight per day for 15 days. At the end of experiment histological and ultrathin sections of pancreas prepared. In diabetic mice disturbances in pancreatic arrangement is observed. While in electron microscopic study pyknotic nuclei, vacuolation and morphological changes in mitochondria, endoplasm reticulum and degranulation of β cells were observed. After treatment with fenugreek repair in pancreatic arrangement especially in β cells was clearly observed. In conclusion fenugreek seed extract could normalize the diabetes and provides protection to pancreatic tissues from damaging effect of diabetes.
M.V. Walvekar*, S.B. Pol and .K. Chandrasekhar Sagar
Department of Zoology, Shivaji University , Kolhapur, Maharashtra, India
31 January, 2014
24 March, 2014
03 May, 2014
01 July, 2014