HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF RIFABUTIN IN BULK AND CAPSULE DOSAGE FORMAbstract
A selective, accurate, HPLC method was developed by this study for the determination of rifabutin in bulk and capsule dosage form. This method was developed by SHIMADZU LC-2010 HT using C18 column in solvents methanol: acetonitrile: ammonium acetate buffer (50: 45: 05) as mobile phase. At 1.0 ml/min flow rate the mobile phase was pumped, and the sample was detected at 278 nm. For standard rifabutin the retention time was 4.8 min. The method was validated for analytical standards such as linearity, accuracy, precision, and robustness. In a wide range of 5-25 (µg/ml) the linearity was observed. The method was validated, and a recovery study indicates accuracy of this method.
R. J. Sajini *, S. Prema, S. Niveditha, S. Nithya, G. M. Pavithra and V. Nivetha
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India.
11 April 2019
21 August 2019
02 September 2019
01 January 2020