HPLC METHOD DEVELOPMENT OF AMLODIPINE BY RP-HPLC IN ITS BULK DOSAGE FORMSAbstract
A new, simple, precise, sensitive, accurate and reproducible Reverse phase HPLC method was developed and validated for the analysis of amlodipine in bulk dosage forms. The separation was conducted by using c-18 RP-HPLC coloumn, which was maintained at ambient temperature. The mobile phase consisting of Phosphate buffer and Acetonitrile (90:10v/v) was delivered at a rate of 1.5 ml/min. The analysis was detected by using UV detector at the wave length of 225nm.The method is validated for its specificity, precision, accuracy, linearity and robustness. The method was found to be linear over the concentration range 10-100 mg/ml (r2 =0.999). The retention time for amlodipine was found to be 3.34min. Limit of quantification of the method is 0.179mg/ml and limit of detection is 0.054 mg/ml.
V. Naveen Kumar, K. Kiran kumar, P. Ramesh Babu, K. Anilkumar
Brown’s College of Pharmacy, Khammam-507 305, Andhra Pradesh, India
06 February, 2012
22 March, 2012
28 May, 2012
01 June, 2012