HPLC METHOD FOR ESTIMATION OF VALTRATE IN VALERIANA JATAMANSI AND VALERIANA PYROLAEFOLIA
AbstractValtrate is one of the major valepotriates reported from various species of the genus Valeriana. Aim of the present study was to develop and validate analytical method for quantification of valtrate in dried roots of Valeriana jatamansi and Valeriana pyrolaefolia, both members of family Valerianaceae. Acetonitrile and water (70:30) in isocratic mode were used with an aflow rate of 1.5 mL/min (at 254 nm) using Waters HPLC system. Valtrate elutes within 10 min. It has shown a linear range from 100-200 ng with a correlation coefficient of 0.999. The reverse phase HPLC method is simple, accurate, precise and can be used for estimation of valtrate in V. jatamansiand V. pyrolaefolia. The studies also show the variation of valtrate content in V. jatamansi, procured from market, wild and cultivated sources.
Article Information
10
1120-1124
559 KB
618
English
IJPSR
Ashwani Kumar *, Maninder Karan and Karan Vasisht
University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
bashwani@pu.ac.in
26 May 2021
13 July 2021
16 July 2021
10.13040/IJPSR.0975-8232.13(3).1120-24
01 March 2022
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