HPTLC DENSITOMETRIC QUANTIFICATION OF SECONDARY METABOLITES FROM POLYCARPAEA CORYMBOSA LAMS
Abstract| Polycarpaea corymbosa has been used traditionally as medicine for the treatment of various ailments. In the present investigation an attempt has been made to quantify phenol, flavonoid and steroid in methanol extract of aerial and root parts of P.corymbosa Lam. by HPTLC method. Different compositions of the mobile phase for HPTLC analysis were tested in order to obtain high resolution and reproducible peaks. The result of HPTLC analysis authenticates the presence of phenols, flavonoids and steroid with several peaks having different Rf values. Our results revealed that at least three types of phenols were seen in aerial methanolic extract and one in root extract. Similarly five types of flavonoids and three types of steroids were found in the Polycarpaea corymbosa whole plant methanolic extract. Phenol and flavonoid were found to have higher concentration in the methanolic extract when compared to steroids. Therefore, from the above results, methanolic extract of P.corymbosa can be used as therapeutic agents to treat various disorders caused by free radical and chemical substances due to presence of its secondary metabolites. |
Article Information
28
2834-2840
622
1454
English
IJPSR
Sindhu Subramanian* and S. Manorama
PG and Research Dept. of Botany Kongunadu Arts and Science College (Autonomous), Coimbatore-29, Tamil Nadu, India
ssindhu389@gmail.com
24 January, 2014
04 March, 2014
07 April, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(7).2834-40
01 July, 2014
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