HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF NAPROXEN SODIUM AND SUMATRIPTAN SUCCINATE IN PHARMACEUTICAL DOSAGE FORMAbstract
A rapid and simple high performance thin layer chromatography (HPTLC) method with densitometry at 230 nm was developed and validated for simultaneous determination of Naproxen sodium and Sumatriptan succinate from pharmaceutical preparation. Separation was performed on aluminum-backed silica gel 60F254 HPTLC plates as stationary phase and using a mobile phase comprising of methanol:distilled water:formic acid in the volume ratio of 0.5:7.5:0.1 (v/v/v), respectively. After development, plates were observed under UV light. The detector response was linear in the range of 200-1200 ng/spot and 100-1000 ng/spot for Naproxen sodium and Sumatriptan succinate. The validated lowest limit of detection was 85 ng/spot and 40 ng/spot whereas lowest limit of quantification was 200 ng/spot and 100 ng/spot for Naproxen sodium and Sumatriptan succinate, respectively. The percentage assay of Naproxen sodium and Sumatriptan succinate was found between 99.25 and 98.03 % respectively. The described method has the advantage of being rapid and easy. Hence it can be applied for routine quality control analysis of Naproxen sodium and Sumatriptan succinate from pharmaceutical preparation and stability studies.