HPTLC QUANTIFICATION OF N-OCTACOSANOL IN TINOSPORA CORDIFOLIA MIERS. STEM OBTAINED FROM DIFFERENT GEOGRAPHICAL SOURCESAbstract
n-octacosanol is a normolipidemic aliphatic primary alcohol obtained from the plant Tinospora cordifolia Miers. In the present study, the plant stems were collected from five accessions namely Anniyalam, Chennai, Hassan, Vishakhapatnam and Bangalore between June and August. Later a simple HPTLC method has been established for determination of n-octacosanol in methanolic extracts of all these geographical sources using Toluene: Ethyl acetate: Formic acid (4.5:4.5:1 v/v) as mobile phase. Detection and quantification were performed by densitometric scanning at λ = 555 nm. The results of HPTLC estimation showed variation in n-octacosanol content in all the accessions. The extract from Anniyalam accession showed significantly higher amount of n-octacosanol (6.54%) and that of Vishakhapatnam showed the least (2.28%). Thus the present work is helpful in selecting Tinospora cordifolia yielding highest percentage of n-octacosanol.
S. Shashidhara* and N. Nandini
Department of Pharmacognosy, Government college of Pharmacy, Bangalore, Karnataka, India
27 April, 2011
06 June, 2011
12 July, 2011
01 August, 2011