HPTLC QUANTIFICATION & PHYTOCHEMICAL INVESTIGATION OF ROOTS OF ACACIA CATECHU WILLDAbstract
Present work has been undertaken to establish the necessary pharmacognostic standards and phytochemical evaluation of roots of Acacia catechu Willd. Various morphological parameters of fresh as well as shade dried form of the roots were studied. Microscopy shows the presence of medullary rays, starch grains, pith, phloem fibres, cork cells and xylem cells. Physico-chemical constants such as Fluorescence analysis of root powder and extracts, ash values, loss on drying, extractive value, swelling index, percentage extractive values for successive extracts, consistency and color of different extracts under ordinary and UV light were evaluated. Phytochemical screening of total ethanolic and aqueous extracts shows the presence of flavonoids, tannins, saponin glycosides, & reducing sugars. HPTLC was carried out for quantification of quercetin in ethanolic extract of the roots of A. catechu Willd. It was concluded that plant contains various phytochemicals; among these tannins and flavonoids are its main constituents.
Jyoti Alambayan *, Manisha Vats and Satish Sardana
Department of Pharmacognosy and Phytochemistry , International Institute of Pharmaceutical Sciences, Vidyagram, Jhundpur, Sonepat, Haryana, India
30 November, 2014
18 February, 2015
21 May, 2015
01 July, 2015