HYPOACIDIC ACTIVITY OF THE HERBAL EXTRACT; A STUDY ON THE INHIBITION OF PARIETAL CELL K+/H+ ATPase PUMPAbstract
Gastrointestinal tract disorders occur due to an imbalance of gastric acid secretion and defensive factors. Histamine receptor blockers and proton pump inhibitors are the most prominent therapies in the treatment of GI tract disorders. Inhibition of the K+/H+ ATPase pump blocks the secretion of gastric acid (HCl) from the parietal cells. The adverse effects of currently available drugs have obligated the screening of herbs as potent alternate medicines. In addition, plants contain different phytoconstituents, which are responsible for increasing defensive mechanisms against GI tract ulcers. Herein, we screen for the inhibition of K+/H+ ATPase pump using the methanolic extract of Clerodendrum inerme leaves. The extract exhibited 62.9% of inhibition (250 μgm) in a dose-dependent manner with an IC50 of 20.560 μgm/ml. Omeprazole (0.2mg/ml) was used as a positive control for the study. GC-MS analysis confirmed the presence of Phytol, a diterpenoid, as a major constituent in the methanolic extract. Hence, terpenoids could be attributed to the hypoacidic activity by inhibiting K+/H+ ATPase enzyme.
S. S. Bandekar and J. E. Nagamani *
School of Sciences, Garden City University, Bangalore, Karnataka, India.
21 February 2020
21 June 2020
28 June 2020
01 February 2021