IDENTIFICATION AND CHARACTERIZATION OF NOVEL PHYTOCONSTITUENTS FROM THE FERMENTED EXTRACT OF LEAVES OF MORINGA OLEIFERA BY GC-MS ANALYSISAbstract
Moringa oleiferais a well-known plant that can be used for the treatment of various disorders or nutritional deficiencies; all parts of this plant possess various activities such as antitumor, antipyretic, antiepileptic, anti-inflammatory, antiulcer, antispasmodic, diuretic, antihypertensive, cholesterol-lowering, antioxidant, antidiabetic, hepatoprotective, antibacterial and antifungal activities and are being employed for the treatment of different ailments. Numerous bioactive compounds were found to be present in the fermented extract of Moringa oleifera with therapeutic activity having great research value by pharmaceutical industries. The fermented extract of Moringa oleifera leaves was analyzed by gas chromatography-mass spectroscopy for the identification and characterization of phytoconstituents and their therapeutic efficacy. The existing compounds identified in extract were Diethyl Phthalate (56.05%), dl-Mevalonic acidlactone (10.40%), 4H-Pyran-4-one, 2, 3-dihydro-3, 5-dihydroxy-6-methyl (8.88%), Bis(2-Ethylhexyl) phthalate (2.99%), 5-Fluoro-6-methyl-5-hepten-2-one (2.42%). Beta-D-,glycopyranoside, methyl (1.94%), glyceraldehydes (1.71%), 2-propen-1-one, 3-[(1,1-dimethylethyl) thio]-1(1.67%), 1,2,3-propanetriol, diacetate 1.16%), 2,4-dihydroxy-2,5-dimethyl-3(2h)-furan-3-one(1.12%), 2-cyclopenten-1-one, 2-hydroxy-(0.99%), 4-(1-hydroxy-ethyl). gamma. Butanolacton (0.98%), 1,3,5-triazine-2,4, 6-triamine (0.92%), 2(3h)-furanone, 5-acetyldihydro(0.86%), dihydro-5-(1-hydroxyethyl)-2(3h)-furanone (0.85%), docosyl ethyl ether(0.77%), benzeneethanol, 4-hydroxy(0.67%), benzene acetic acid (0.61%), benzene, 1,1′-sulfonylbis-(0.59%), 2-propanamine, n-methyl-n-nitroso (0.59%), 1-heptanol(0.58%), 3,4,5-trimethoxyphenol (0.41%), phenol, 5-ethenyl-2-methoxy (0.41%), 2-oxepanone (0.37%), 3,3-dimethyl-1-(2-naphthyl) butanone (0.31%), furaneol (0.28%), 3(2h)-furanone, 4-hydroxy-5-methyl(0.24%), butanoic acid, 3,3-dimethyl (0.19%), 2-propanamine, n-methyl-n-nitroso (0.08%). These compounds were established qualitatively via GC-MS evaluation. GC-MS reports will be auspicious in the preparation of herbal nutraceuticals, cosmeceuticals and the pharmaceutical area.
Avneesh Kumar and Akash Ved *
Goel Institute of Pharmaceutical Sciences, Near Indra Canal, Ayodhya (Faizabad) Road, Lucknow, Uttar Pradesh, India.
04 August 2022
07 September 2022
21 October 2022
01 April 2023