IDENTIFICATION AND ISOLATION OF BIOACTIVE COMPONENTS USING GC-MS, UV VISIBLE, FTIR, HPLC, HPTLC AND ANTIOXIDANT ACTIVITY PRESENT IN ETHANOLIC EXTRACT OF INDIAN SPICES MIXTURE

Introduction: The spices, despite their taste and flavor, have certain therapeutical uses from ancient days. For this research work, we have chosen some selected spices indefinitely amount. Materials and Methods: Phytochemical analysis, mineral estimation Characterisations such as GC-MS, UV Visible & FTIR spectroscopy, HPLC, HPTLC, and Antioxidant activity were carried out. Results & Discussion: Results of the research have shown that the phytochemicals like Flavonoids, Phenols, and Proteins were found in abundance, Alkaloids, Saponins, Terpenoids, Anthocyanins, Carbohydrates, Xanthoproteins, and Cardiac glycosides are moderately present in the mixture extract and Tannins, Phlobatannins, Coumarins, Emodin, Lekoanthocyanin, Anthroquinone, steroid, Glycosides are present in trace amount. In this study, minerals such as Iron, Sulphur, Manganese, Sodium, Organic carbon, and Zinc were abundantly present in the spices mixture extract. Copper and Phosphorus were moderately present. Calcium, Potassium, Magnesium, Nitrogen, Boron, Selenium, and Molybdenum were present in trace amounts in the mixture extract. Spices mixture extract is a rich source of antioxidant activity. The DPPH assay and total antioxidant activity of spices were carried out, and the results were obtained, indicating the strong antioxidant activity. Through GC-MS, 45 compounds were obtained, and in these 12 compounds were proven biologically active using Dukes database. Functional groups present in the extract were identified using UV Visible and FTIR Spectroscopy was done to identify the σ-bonds, π-bonds, and lone pair of electrons of the identified components. HPLC was carried out to determine the predominant flavonoids like Quercetin, Rutin, Gallic acid, and Catechin. HPTLC was performed to estimate the Quantity of the flavonoids, especially Quercetin and Rutin. Conclusion: Due to Flavanoids, Minerals and Antioxidants, this study proves to have a strong curative effect against respiratory infections.