IDENTIFICATION AND QUANTIFICATION OF OLEANOLIC ACID FROM CYCLEA PELTATA BY HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) METHOD

Objective: To identify and quantify oleanolic acid from the roots of cyclea peltata by HPTLC method. Methods: The Plant cyclea peltata is a perennial herb from Menispermacae family. The powdered roots of plant cyclea peltata were extracted with methanol. Methanolic extract prepared separated with n-butanol, chloroform, and ethyl acetate as total saponin, total alkaloid and phenolic compound fractions respectively. The chemical characterization of oleanolic acid was done by High-Performance Thin Layer Chromatography (HPTLC) in a mixture of toluene: methanol (9:1) as a mobile phase which developed in anisaldehyde sulphuric acid reagent. Result: The extraction yield of total saponin fraction was 4.3% (w/w). The R_f value 0.31 found to same as the value of standard oleanolic acid. Linear regression analysis data for the calibration plots showed a good linear relationship between concentration (1-6 μg) R (r²) = 0.992408 concerning peak height and area. The limit of detection and limit of quantification of Oleanolic acid were detected to be 10.75 μg and 40.70 μg per spot. The amount of oleanolic acid in the methanol extract of roots was found to be 3.442 μg. Conclusion: Based on HPTLC result and its interpretation, it can be concluded that oleanolic acid present in roots of cyclea peltata which give identical, characteristic signals and absorbance similar to earlier reported reference standards.