IDENTIFICATION OF E.COLI NISSLE 1917 PROTEINS BY USING 2-D GEL ELECTROPHORESIS UNDER THE INFLUENCE OF COCOS NUCIFERA SAP AND WINEAbstract
Proteomics is the large scale study of proteins. Before going to analyse the differentially expressed proteins, through 2-D gel electrophoresis the proteins were extracted in a sample under stress. In the present study, we were isolated the protein samples of E. coli Nissle 1917 treated with cocoti sap and wine stress. The isolated proteins washed with 2-D clean up kit because it was very sensitive to salts and detergents. Quantify the protein concentration for loading the samples in 2-D electrophoresis. Proteins were separated by based on IEF and Molecular weight. By using Image master 2-D platinum 6.0 software programmes analyze the protein spots in the gel. We noticed over all 800 proteins in our gel, 370 protein spots were visualized clearly, in that considers 15 spots were isolated based on the regulation. Ten spots shows up regulation and remaining shows down regulation. One newly expressed protein was isolated from wine treated gel when compared to the control and also sap treated gels. Independent t-test was performed to analyze the significant difference between up and down regulation values. Further, this study helps to undergo the proteins identification and its functions through MALDI-TOF analysis.
K. Chandrasekhar, S. Sreevani and J. Pramoda Kumari*
Dept. of Microbiology, S.V.University, Tirupati, Andhra Pradesh, India
20 January, 2014
06 March, 2014
05 April, 2014
01 July, 2014