IDENTIFICATION OF INTER SPECIES GENETIC VARIABILITY BETWEEN TWO MORPHOLOGICALLY SIMILAR SPECIES OF WITHANIA THROUGH PROTEIN AND RAPD MARKERSAbstract
The two species of Withania i.e. Withania obtusifolia and Withania somnifera were usually misinterpreted because of their co-existence in mixed populations. The present investigation involves analyzed of interspecific relationship between these two species. A careful morphological, anatomic and phytochemical, identification of the two species has led to the findings that there was a slight variation between these two species. SDS-PAGE has been effectively used for the comparative study of proteins profiles in leaf and seeds of W. somnifera and W. obtusifolia. The RAPD analysis of W. somnifera and W. obtusifolia encouraged us to think positively that there are most considerable genetic variations between these two species. This result was the most important evidence for differences between the two Withania species.
A. Ramachandran, M. Senthil Kumar , K. Paneerselvam , D. Vinothkumar and A. Shajahan
Climate Change and Adaptation Research, Anna University 1, Guindy Campus- 620 025, Tamil Nadu, India
18 March, 2013
21 April, 2013
21 June, 2013
01 July, 2013