IMMOBILIZATION AND ESTIMATION OF ACTIVITY OF YEAST CELLS BY ENTRAPMENT TECHNIQUE USING DIFFERENT MATRICESAbstract
Background: It becomes clear that living processes were based on the action of enzymes. Cell immobilization serves as a useful tool for the immobilization of intracellular enzymes. The purpose of study is to improve economically important processes by attaching the yeast cells to matrices. Methods: In this analytical study by using mixed models of matrices like (sodium alginate alone, sodium alginate and gelatin, sodium alginate and agar, sodium alginate and glutaraldehyde) to determine the effective combination for immobilizing cells without any damage. Results: It was noted that the glucose content in both cells increased which indicates the production of carbon dioxide and alcohol and then the values started falling down this is because the osmotic concentration of the sugar gets so great that the yeast is unable to get enough water for growth. Conclusion: Glucose utilization was analyzed in free and immobilized cells on four different matrices for verifying their performance and the improvement of immobilized yeast cells over free yeast cells. Use of mixed matrices enhanced the biological activity of cells thus improved their performance. Immobilization of yeast cell showed technical and economic advantages over free cell system.
N. K. D. Devi * and A. S. S. Nagamani
KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India.
22 September, 2017
15 December, 2017
25 June, 2018
01 July, 2018