IMMOBILIZATION OF TANNIN ACYL HYDROLASE FROM ASPERGILLUS NIGERAbstract
Tannin acyl hydrolase, commonly referred to as tannase (E.C. 22.214.171.124), an inducible extra-cellular enzyme produced by a number of animals, plants and microbes. In this investigation, tannase production under solid-state fermentation by using Aspergillus niger and the waste residue of cashew husk was used as substrate for obtaining the desired fermented product. After termination of fermentation gallic acid was isolated from the tannase, and it was used in the production of an antibacterial drug Trimethoprim by using chemical and bioconversions. Microbial tannase is more stable than tannase from other sources like plants or animals. Tannase from fungal sources are reported to be active in a wide range of pH and temperature. Aspergillus niger was used in this study for the immobilization of tannase enzyme. Tannase production was reached maximum within 24 to 36 h against crude tannin extract obtained from Anacardium occidentale by using Aspergillus niger. Tannase assay was calculated. Activation energy of the immobilized enzyme was lower than that of the free enzyme. Optimum pH and temperature was evaluated for the free and immobilized enzyme.
B. Lenin Kumar*, N. Lokeswari and D. Sriramireddy
Research Scholar, Department of Biotechnology, Dr. B.R. Ambedkar University, Etcherla, Srikakulam Dist., Andhra Pradesh, India
27 December, 2012
28 January, 2013
15 March, 2013
01 April, 2013