IMPORTANCE OF RP-HPLC IN ANALYTICAL METHOD DEVELOPMENT: A REVIEWAbstract
Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. Reversed phase chromatography has found both analytical and preparative applications in the area of biochemical separation and purification. Molecules that possess some degree of hydrophobic character, such as proteins, peptides and nucleic acids, can be separated by reversed phase chromatography with excellent recovery and resolution. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.
Sanjay Kumar D and D.R. Harish Kumar*
Department of Pharmaceutical Analysis, Krupanidhi College of Pharmacy, Sarjapura Main Road, Carmelaram post, Bangalore-560 035, Karnataka, India
25 August, 2012
07 October, 2012
20 November, 2012
01 December, 2012