IN SILICO CHARACTERISATION AND COMPUTATIONAL MODELLING OF NEURO-TOXINS OF INDIAN COBRAAbstract
Snake venom is cocktail of various enzymes, toxins. Snake venom components have major role as therapeutics application in current drug industry. Snake venom toxins plays vital role as one of the major component in case of bite and most of the toxins are lethal which are of various types such as cardio-toxins, myotoxins and neurotoxins etc. The Indian Cobra neurotoxins sequences were retrieved form Swissprot Database. The ten neurotoxins were characterised and three were modelled using in silico approach whose structure were not available on Protein Data Bank and protein model portal. The neurotoxins were modelled using homology modelling approach and energy minimisation was carried out for all the three neurotoxins. The stearic hindrance was removed using chiron server. The Ramchandran plot was used to validate the modelled structure which provides idea of the modelled structure conformation and configuration and it was satisfactory. Further the modelled structures will be used to understand docking with the suitable receptor.
L R. Samant*, N. Shah, D. Thatavarthy M. Ghag-Sawant and A. Chowdhary
Systems Biomedicine Division, Venomous Animal Unit, Haffkine Institute for Training, Research and Testing, Acharya, Donde Marg, Parel, Mumbai - 400012, Maharashtra, India
19 February, 2014
22 April, 2014
18 June, 2014
01 August, 2014