IN-VITRO AND IN-VIVO STUDIES OF PHB CAST FILMS BLENDED WITH VARIOUS HERBS AND ITS BIOCOMPATIBLE CHARACTERIZATION

The present study deals with the PHB made cast film with different plant and algal formulations. Its biocompatibility was tested in-vitro and in-vivo. The anti-inflammatory activity was higher in F4 and F5, close to the standard Diclofenac. The F7 and F8 exhibited moderate cytotoxicity, and formulation F2 exhibited low cytotoxicity. The maximum zone of inhibition was exhibited in F5 followed by F4 against skin pathogens. The overall results of antioxidant activity indicated that F4, F5, and F8 have good antioxidant properties. Percentage (%) of wound contraction at the 10^th day of control was 16.9% and PHB alone was 21.3%. F5 and F3 showed higher activity with 51.7 and 49.7%, respectively. The self-healing potential was found to be 35.6%, but PHB was able to cure additional 5% (40.5) on 16^th day. F5, F3, and F4 showed high activity of 81.5, 80.1, and 79.9, respectively. The in-vitro and in-vivo studies showed that the Centella asiatica and Padina tetrastromatica with PHB has an effective activity towards antimicrobial, antioxidant, and wound healing activity. In order to identify the positive correlation between in-vitro and in-vivo study a multiple regression was performed. Data of the experiments were fitted to the model and supported by the multiple correlation coefficients (R²) and the correlation of determination (adjusted R²). The model was fit and statistically significant with the p-value (< 0.01) and the indication of positive regression between in-vitro and in-vivo. Thus in-vitro and in-vivo studies using Centella asiatica and Padina tetrastromatica showed positive and significant correlation.