IN-VITRO ANTHELMINTIC EFFECTS OF MEDICINAL PLANT CASSIA FISTULA EXTRACT ON THE TEGUMENT OF FASCIOLA GIGANTICA BY LIGHT MICROSCOPE

The efficacy of alcoholic Cassia fistula leaves extract on the tegument of Fasciola gigantic was studied by light microscopy. Live liver fluke Fasciola gigantica parasites were collected from the infected liver and bile duct of freshly slaughtered domestic goat (Capra hircus) at the local zoo abattoir in Udaipur. Liver flukes F. gigantica were maintained in 0.9% physiological saline and divided into three groups. The first group of the parasite was untreated used as control liver fluke, the Second group of parasites was treated with synthetic drug albendazole and the third group of parasites was given in-vitro treatments with alcoholic extracts of C. fistula leaves. The control and in-vitro treated F. gigantica with the alcoholic C. fistula leaves extract, and flukicide drug albendazole were fixed in Bouin’s fixative for histological study.  The present studies revealed that the alcoholic extract of C. fistula leaves was an effect on the tegument of parasite; showed abounded cuticular disruption, detachments, discontinuous, babblings, damage in tegument and in parenchymal cell and the complete breakdown of cells in the parenchyma, leaving vacuolated areas. Albendazole is less effective than alcoholic C. fistula leaves extract. This study suggests that the alcoholic extract of the leaves of Cassia fistula could offer a suitable and cheaper alternative anthelmintic as a comparison to synthetic drugs. The results of this study will help prepare the eco-friendly, less costly anthelmintic veterinary drug, socio-economic upliftment of cattle farmers.