IN VITRO ANTICANCER ACTIVITY OF BIOPHYTUM SENSITIVUM WHOLE PLANT EXTRACTS AGAINST CERVICAL AND LIVER CANCER CELL LINESAbstract
In the present study, the various extracts viz aqueous, ethanol, chloroform and Petroleum ether of Biophytum sensitivum whole plant were evaluated for the anticancer activity against cancer cell lines. The cervical (Hela) and liver cancer (HepG2) cell lines were used to screen the in vitro anticancer challenging with the B.sensitivum extracts. Concentration ranges from 20 to 100 microgram of B.s crude extracts were screened against Hela and HepG2 cells. The antiproliferative activity of B.sensitivum were performed by 3-[ 4,5–Di methyl thiazol-2-yl]-2, 5-diphenyltetrazolium Bromide (MTT) assay. The cytotoxicity was highly significant in the ethanolic extract and chloroform extract than the aqueous and petroleum ether extract. The maximum non toxic concentration was observed at 150µg concentration of aqueous extract in the vero cell line.
M. P. Shanthi, G. Bupesh * , S. Magesh, K. Meenakurmari, K. Saravanan and N.S. Muthiah
Central Research Laboratory, Sree Balaji Medical College and Hospital (SBMCH), Bharath University, Biher, Chrompet, Chennai, Tamil Nadu, India
27 May, 2016
22 October, 2016
18 November, 2016
01 December, 2016