IN-VITRO ANTICANCER POTENTIAL OF CHLOROFORM EXTRACT OF LEAVES OF THULASI VETTILA- A CULTIVAR OF PIPER BETLE L. IN KERALA

The present study was designed to evaluate the anticancer potential as well as cytotoxity of Chloroform extract of Thulasivettilacv of Piper betle L. is an evergreen perennial creeper belonging to family Piperaceae, and known to possess numerous medicinal properties. Current study focuses on evaluating antiproliferative potentialities of Betel leaves. The antiproliferative effect was tested against two cancer cell lines HeLa and HT 29, while cytotoxity was tested against in a normal myoblast cell line, L6 by MTT assay. Five different concentration of the chloroform extract were used for this study. Qualitative phytochemical analysis of leaf extracts showed the presence of tannins, alkaloids, phenols, flavonoids and glycosides. HeLa cell lines exhibited less antiproliferative activity 25.8 µg/ml ± 0.05% viability at 100µg/ml of extract by MTT assay and the IC₅₀ value was found to be 45.19 µg/ml. In case of HT 29 colon cancer cell line, chloroform extract of plant showed 30.32 ± 0.04% µg/ml viability compared with the L6 normal cell line was24.00 ± 0.08% µg/ml. The IC₅₀ values were found to be 56.43 µg/ml and 30.26 µg/ml respectively. The extract showed dose dependent anticancer activity. The results revealed that the extract is more potent against HeLa cell lines than HT 29 cell lines.