IN-VITRO ANTIFUNGAL STUDY OF FILTRATES OBTAINED FROM SOME MARINE FUNGI AGAINST SOME DERMATOPHYTES AND YEASTSAbstract
Many infectious diseases have been treated with natural medicine. Now, fungi metabolites continue to play a major role as a therapeutic medicine in many countries. The present study reveals the potentiality of filtrate of Aspergillus terreus and Cladosporium cladosporioides as antidermatophitic agents. Molecular identification of isolates fungi using DNA sequencing of EF4 and ITS4primerswere performed. The fungal filtrate of A. terreus and C. cladosporioides were prepared in Potato dextrose broth and Sabouraud dextrose broth media. Antidermatophitic activity of it was evaluated against Trichophyton mentagrophytes, Trichophyton verrucosum, Microsporum gallinae, Microsporum gypseum, Microsporum canis, Microsporum floccosum, Candida albicans, and Candida tropicalis using dry weight method. The results show that E. floccosum was the most sensitive for Sabouraud dextrose broth filtrate of A. terreus while M. gallinae was the most sensitive for Potato dextrose broth of A. terreus. The potato dextrose broth filtrate of A. terreus was further subjected to the determination of the Minimal inhibitory concentration using different concentrations. The minimal inhibitory concentration values of different extracts were found to be different but in the range of (2.5 – 10 mg/ml), and defined the Anti-scavenging activity and Total phenolic contents for both fungal filtrates. Potato dextrose broth filtrate of A. terreus has the highest anti scavenging activity (71.5 %) and the highest amount of phenolic content (30.08mg/gdw). In addition, some bioactive compounds from fungal filtrates are separated and estimated by using High-performance liquid chromatography.
M. AL-Kattan, E. N. Danial *, W. A. Alshehri and N. A. Alzumay
Department of Biochemistry, Faculty of Science, University of Jeddah, Saudi Arabia.
25 November 2019
18 December 2020
17 January 2021
01 February 2021