IN-VITRO ANTIFUNGAL SUSCEPTIBILITY REVEALS OCCURRENCE OF AZOLE AND ALLYLAMINE RESISTANCE AMONG CLINICAL ISOLATES OF CANDIDA ALBICANS AND CANDIDA NON ALBICANS FROM CENTRAL INDIAAbstract
Background: Drug resistance among Candida species constitutes the most significant problem in the treatment of Candidiasis.Systematic studies on antifungal drug susceptibility which may be useful in deciding clinical strategies are not routinely done in India and other developing countries. Objective: Aim of this study was testing sensitivity of clinical isolates of Candida albicans and non albicans to frequently prescribe antifungal drugs, fluconazole, ketoconazole, Itraconazole, Amphotericin B and Terbinafine. Material and methods: 25 strains of C. albicans and non albicans were tested in vitro for susceptibility to five antifungal agents, by using standard broth macro dilution method (CLSI M 27-A). Results: The present study revealed the percentage and extent of emerging drug resistance and cross resistance among Central Indian clinical isolates of C. albicans and non albicans against azoles and allylamine. In the present study, the total percentage of resistance was found to be 84% (21/25). The drug for which maximum resistance were found was Ketoconazole (64%) followed by Itraconazole (44%) Terbinafine (24%) and lastly Fluconazole (20%). The total percentage of cross resistance was 62% (13/21) and the maximum seen in C. albicans followed by C. glabrata, C. krusei and C. guilliermondi. No resistance was found for polyene drug Amphotericin B. Conclusion: This short study from India has exhibited the increasing frequency of resistance and Cross resistance of C.andida species against azoles and allylamine. We suggest a comprehensive study to determine the extent and degree of antifungal drug resistance among Candida species in India.
Shruti Singh *, Shesh R. Nawange and Nidhi Warthe
Department of Biotechnology, Mithibai College, Vile parle (W), Mumbai - 400056, Maharashtra, India.
04 May, 2014
07 August, 2014
14 August, 2014
01 December 2014