IN VITRO ANTILEISHMANIAL ACTIVITY AND GC-MS ANALYSIS OF ETHYLACETATE FRACTION OF ALLIUM CEPA (EAFAC)Abstract
In this study, GC-MS analysis shown that ethyl acetate fraction of Allium cepa (EAFAC) (band one) consists of 55 compounds which shown antileishmanial activity. (IC50 73.1 ± 2.1 ug/ml) is the inhibitory concentration which is observed for ethyl acetate fraction of Allium cepa (EAFAC) (band one). Leishmania major is the specie used for this leishmanicidal assay. In this bioassay, Amphotericin B (IC50 = 0.29 ± 0.05 ug/ml) and pentamidine (IC50 = 5.09 ± 0.09 ug/ml) were used as positive control drugs to compare the parasite inhibition with that ethyl acetate fraction of Allium cepa (EAFAC) (band one). This activity was perceived under incubation period 72 hours and incubation temperature 22 °C. Methanol extract of Allium cepa (MEAC), Hexane fraction of Allium cepa (HFAC), Ether fraction of Allium cepa (EFAC), and Diethyl ether fraction of Allium cepa (DEFAC) and butanol fraction of Allium cepa (BFAC) exhibited no antileishmanial activities. In this research paper we represent 30 compounds out of 55 compounds of ethyl acetate fraction of Allium cepa (EAFAC) (band one).
J. K. Achakzai*, M. A. Panezai , A. M. Kakar, Z. Agha, J. Khan and A. Kakar
Institute of Biochemistry, University of Balochistan, Quetta, Pakistan.
18 February, 2017
22 April, 2017
27 May, 2017
01 October, 2017