IN VITRO ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF BARK OF CORDIA DICHOTOMA FORSTAbstract
In this study, the methanol extract of bark of Cordia dichotoma Forst., belonging to the family Boraginaceae was prepared by successive extraction procedure ( petroleum ether, ethyl acetate, methanol) in soxhlet apparatus and subsequently, the extract was evaluated for its phytoconstituents and pharmacological activity. Comparatively, higher yield (7.11%) of methanol extract was obtained. Phytochemical tests revealed the presence of alkaloid, glycosides, proteins, amino acids, phenolic compounds, flavonoids, tannins and saponins in the extract. The methanol extract was evaluated for its in vitro antioxidant property by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical assay; sodium nitroprusside generated nitric oxide (NO), hydrogen peroxide (H2O2).Total phenolic content and total flavonoid contents were also carried out. The extract showed IC50 values of 62.46 µg/mL, 48.75µg/mL, 63.5 µg/mL for DPPH free radical, total phenolic content and flavonoid content, respectively. The current study is carried out to investigate the free radical scavenging potential of methanolic extract of bark of Cordia dichotoma using in-vitro models. These models demonstrate antioxidant activity in a concentration dependent manner.
Nazim Hussain, B.B. Kakoti and Shashi Alok
Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh-786004, Assam, India
03 August, 2013
27 September, 2013
15 December, 2013
01 January, 2014