IN-VITRO ANTIOXIDANT ACTIVITY OF SOLANUM SISYMBRIFOLIUM AERIAL PARTS

Objective: Recently, natural plants have received much attention as sources of biological active substances including antioxidants. In the present study we investigated In-vitro antioxidant activity of hexane, ethyl acetate and ethanol extracts of Solanum sisymbrifolium aerial parts. Methods: In-vitro antioxidant activity was evaluated for extracts by using free radicals Superoxide (Riboflavin photo reduction method) and DPPH (The scavenging activity for DPPH free radicals was measured according to the procedure described by Bracaand Sanja)^. Results: The selected plant extracts produced concentration dependent percentage inhibition of different free radicals and produced maximum activity at a concentration of 800µg and there after the percentage inhibition was raised gradually to its maximum level with higher concentrations. Conclusion: In the present study we found that the extracts of Solanum sisymbrifolium showed good antioxidant activity. Among the three extracts, hexane extract showed better activity than the other extracts on the tested super oxide free radical. The order of activity is in the following manner: Ascorbic acid >hexane extract > ethyl acetate extract> ethanol extract. Ethanol extract showed better activity than the other extracts on the tested DPPH free radical. The order of activity is in the following manner: ethanol extract >Ascorbic acid > ethyl acetate extract > hexane extract.