IN-VITRO ANTIOXIDANT AND ANTIDIABETIC ACTIVITY OF CUMINIC ACIDAbstract
Diabetes mellitus is a metabolic disorder caused due to lack of insulin and/or its insufficiency. It is ranked ninth among the leading causes of death globally. As Oral hypoglycemic agent usage causes one or more side effects, phytochemicals can be used as alternative herbal medicine to treat diabetes mellitus with little or no side effects. In the current study, Cuminic acid was evaluated for its antioxidant and antidiabetic activities under in-vitro. Different doses of Cuminic acid were used to analyze free radical scavenging effect by DPPH, H2O2 & NO methods. α- amylase and α- glucosidase inhibitory effects were studied using various doses of Cuminic acid. Glucose diffusion inhibitory assay was carried out using a Dialysis bag. In the results of the antioxidant study, Cuminic acid showed the highest free radical scavenging activity in all the methods compared to standard Ascorbic acid. Enzyme inhibition studies also showed significant increase in antidiabetic activity indirectly with increasing concentrations of Cuminic acid, which was found to be equivalent to the standard drug acarbose. Glucose diffusion inhibitory assay also proved significant compared to the standard metronidazole drug. The study’s results substantiate that Cuminic acid has potent in-vitro antioxidant and antidiabetic activity.
Partha Sarathi Chinthirla, A. Rajasekhar, N. Vedasree, P. Muni Rajeswari and Ch. Appa Rao *
Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
08 May 2022
08 June 2022
10 June 2022
01 July 2022