IN-VITRO ANTIOXIDANT AND IN-VIVO HEPATOPROTECTIVE ACTIVITY OF GANODERMA LUCIDIUM STRAINS DARL-4 & MS-1 AGAINST PARACETAMOL INDUCED HEPATOTOXICITY

The purpose of current research work is to evaluate the in-vitro antioxidant and in-vivo hepatoprotective activity of Ganoderma lucidum strains DARL-4 and MS-1. Antioxidant activity of different extracts was evaluated by using DPPH and ABTS method. Hepatoprotective activity of hydroalcoholic extract of G. lucidum strain DARL-4 and MS-1 were evaluated by paracetamol-induced liver damage in rats. The hydroalcoholic extract of DARL-4 and MS-1 were orally administered to the animals at a dose of 300 mg/kg once daily for 14 days. On the 14^th day the paracetamol (2 g/kg) was also orally administered. The degree of protection was measured by estimating the serum biochemical parameters. The result showed that the hydroalcoholic extract of DARL-4 and MS-1 showed potent antioxidant activity than the ethanolic, methanolic and aqueous extract. The serum biochemical parameters which were elevated due to paracetamol toxicity were significantly reduced. The histopathological studies also supported that extracts markedly reduced toxicity of paracetamol. The hydroalcoholic extract of MS-1 showed a more potent hepatoprotective effect than that of DARL-4. The present study revealed that in-vitro cultivated G. lucidum strains DARL-4 and MS-1 have significant free radical scavenging potential and liver protection property.