IN-VITRO ANTIUROLITHIATIC POTENTIAL OF VARIOUS EXTRACTS OF MUCUNA PRURIENSAbstract
The present study was aimed to investigate the methanolic (MEMP), ethyl acetate (EEMP) and n-hexane (HEMP) extracts of the whole plant of Mucuna pruriens for their in-vitro Antiurolithiatic potential. Calcium oxalate crystallization was induced by the addition of 0.01M sodium oxalate solutions in synthetic urine. The in-vitro anti-urolithiatic activity of various extracts of M. pruriens was investigated by measuring the turbidity of various concentrations of (50µg m/ml, 100µg m/ml, 150µg m/ml, 200µg m/ml, and 250µg m/ml) each extract in synthetic urine, by using UV/Visible spectrophotometer at 620 nm after incubating them for 10 min and microscopically under microscope equipped with digital camera at 10X. The results of the present study showed that the MEMP, EEMP dose-dependently inhibited the formation of calcium oxalate (CaOx) crystallization in the synthetic urine which was evident from the spectroscopic as well as in the microscopical studies. It was concluded that the antiurolithiatic activity of Mucuna pruriens was possibly mediated through inhibition CaOX crystals in the synthetic urine. But, further, in-vivo, preclinical studies are required to confirm its potential as Antiurolithiatic in terms of clinical use.
S. Vamsi *, M. Raviteja and G. S. Kumar
Department of Pharmacology, Sree Vidyanikethan College of Pharmacy, Tirupathi, Chittore, Andhra Pradesh, India.
12 March 2014
22 April 2014
07 June 2014
doi: 10.13040/IJPSR.0975-8232.5 (9).3897-02.
01 September 2014