IN-VITRO COMPARATIVE ANTICANCER ACTIVITY STUDY OF METHANOLIC EXTRACT OF TRADITIONALLY USED MEDICINAL PLANT OF MIZORAM

Objective: The present study explores the anticancer potentially of plant sample based on the evidence from the ethnomedicinal practice of the plant. The main objective was to find out the in-vitro comparative anticancer activity of the various methanolic extract. Method: Plants samples such as Mikania micrantha, Allium hookeri, Eryngium foetidium, and Alpinea galanga were collected, identified, and authenticated. By using Trypan blue test Human cervical cancer (Hela), cells were counted. The Human cervical cancer (Hela) cells were treated with methanolic extract of Mikania micrantha, Allium hookeri, Eryngium foetidium, and Alpinia galangal and 20 µL of MTT (5 mg/mL) solution was added to cells per well, and the plate was moved to a CO₂ incubator (3-4 h incubation). Measurement was performed using a Spectramax M2 Microplate Reader (Molecular Diagnostic, Inc.) at a wavelength of 570 nm. Results: The Cytotoxicity of studied medicinal plants of Mizoram such as Mikania micrantha, Allium hookeri, Eryngium foetidium, and Alpinia galanga exhibited cytotoxicity in an increased manner with increase concentration against Hela cells. Their IC₅₀ values were 49.02, 138.5, 199.7, and 209.4 µg mL^-1, respectively. The IC₅₀ of doxorubicin was found to be 3.305 µg mL^-1. The anticancer activity of the leaves related to their contents of flavonoids. This study validates the traditional use of plants in the management of cancer.