IN-VITRO CYOTOTOXIC STUDIES OF CAESALPINIA BONDUCELLA F. USING AQUEOUS EXTRACT OF SEED POWDER AND CALLUSAbstract
In the traditional system of Indian medicine Caesalpinia bonducella Flem. is widely used for its medicinal properties. All parts are valuable as it contains various secondary metabolites. Seeds contain 20-30% of oil. On the basis of phyto-chemical analysis of the seed it is known to contain alkaloids, flavonoids, glycosides, saponins and furano di terpenes. The plant possesses antidiabetic, antioxidant, antitumor, antiproliferative and anti imflammatory activities. “Cytotoxicity” is the ability to kill the cells. In the present study experiments were carried out to check for the cytotoxic nature of seeds and callus using Daphnia cultures. The method used is simple, inexpensive and even a low toxin amounts are sufficient to perform the test. Cytotoxic nature was tested using aqueous extracts of seed powder as well as callus. From the experiments it was observed that extract from seed powder as well as callus expressed cytotoxic activities, however, seed powder extract was more effective compared to callus extract.
Shirsat Rajani Kamerkar Urwashi and Rai Aruna *
Department of Botany, Smt. C.H.M. College (Affiliated to University of Mumbai), Ulhasnagar, Thane, Maharashtra.
17 July, 2014
11 October, 2014
19 January, 2015
01 March, 2015