IN-VITRO CYTOTOXIC ACTIVITIES OF LEAF EXTRACT OF GARDENIA LATIFOLIA AIT. AND GARDENIA GUMMIFERA LINN.Abstract
Plants are the natural reservoir of many anticancer agents. Gardenia latifolia Ait. and Gardenia gummifera Linn. belonging to the family Rubiaceae. Gardenia Ellis. is a genus of 142 species, which occur in tropical and sub-tropical regions of Africa, South Asia, Australasia and oceania. The genus was named by Carl Linnaeus after Dr. Alexander Garden (1730-1791), a Scottish-born American naturalist. Both the plant leaves were extracted by ethanol, extracts were subjected to cytotoxic activity study against MCF-7 cell lines using the MTT assay. MTT assay was the technique utilized for cell survival determination. Measurements were performed and the concentration required for a 50% inhibition of viability (CTC50) was determined graphically. The concentration of ethanolic extract yields the value of CTC50 as 170.00±2.0 µg/ml and 200.00±1.6 µg/ml respectively for Gardenia latifolia Ait. and Gardenia gummifera Linn. The results showed that the Gardenia latifolia Ait. had potential cytotoxicity than the Gardenia gummifera Linn. against MCF-7cell lines.
K. Vindhya and S. Leelavathi
DOS in Botany, University of Mysore - 570 006, Karnataka, India.
23 April, 2014
12 June, 2014
19 July, 2014
01 November, 2014