IN VITRO CYTOTOXIC EFFECT OF METHANOLIC CRUDE EXTRACTS OF OCIMUM SANCTUM
AbstractThe aim of this research is to observe the cytotoxic activities of methanolic crude extracts of Ocimum sanctum (Family: Lamiaceae). The leaves of Ocimum sanctum was extracted with organic solvent (methanol) and the extracts were fractionated by using solvent-solvent partition. The n-hexane, ethyl acetate, and chloroform soluble fractions of methanolic crude extract of Ocimum sanctum were screened for cytotoxic activity using brine shrimp lethality bioassay. A reputed cytotoxic agent, vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay it was well predicted that n-hexane, ethyl acetate, and chloroform soluble fractions of methanolic crude extracts possess cytotoxic principles (LC50 4.36 mg/mL, LC50 5.37 mg/mL and LC50 10.00 mg/mL respectively) comparison with positive control, vincristine sulphate (LC50 0.563 mg/mL).
Article Information
39
1159-1163
598KB
1267
English
IJPSR
Aparna Debnath and Mohammad Musarraf Hussain*
Assistant Professor, Department of Pharmacy, Jagannath University, Dhaka-1100, Bangladesh
m.musarraf.hussain@gmail.com
21 November, 2012
21 February, 2013
28 February, 2013
http://dx.doi.org/10.13040/IJPSR.0975-8232.4(3).1159-63
01 March, 2013
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