IN VITRO CYTOTOXICITY OF MADHUCA INDICA AGAINST DIFFERENT HUMAN CANCER CELL LINESAbstract
Cancer is a public health problem all over the world. Large number of plants and their isolated constituents has been shown to potential anticancer activity. Ethanolic whole plant extract of Madhuca indica showed in vitro cytotoxicity against different human cancer cell lines such as lung, neuroblastima, and colon. There was no growth of inhibition recorded against liver cancer cell line. Sulforhodamine B dye (SRB) assay was done for in vitro cytotoxicity test assay. The in vitro cytotoxicity was performed against five human cancer cell lines namely of lung (A-549), liver (Hep-2) colon (502713 HT-29) and neuroblastima (IMR-32). The activity was done using 100µg/ml of the extract. Against lung (A-549) cell line plant extract showed 83% growth of inhibition. In case of liver (Hep-2)showed no activity reported, where as in case of colon 502713 cell line plant extract showed maximum activity. In case of HT-29 liver human cancer line and IMR-32 neuroblastima cell line plant extract showed 99% and 98% activity respectively.
Asima Shaban , Satish K. Verma*, Rajesh Nautiyal Santosh Kumar Singh , Reena Purohit and Madhvi Lata Chimata
Lecturer, Department of Zoology, K.L.P.G College, Naini, Allahabad, Uttar Pradesh, India
11 January, 2012
17 February, 2012
19 April, 2012
01 May, 2012