IN-VITRO EVALUATION OF ANTIBACTERIAL ACTIVITY AND CYTOTOXICITY EFFECT OF CHEMICALLY SYNTHESIZED ZnO NANOPARTICLEAbstract
Nanoparticles, generally considered as particle with a size of upto 100nm, exhibit completely new or improved properties as compared to the larger particles of the bulk material that they are composed of based on specific characteristics such as size, distribution and morphology. In this study ZnO nanoparticle was synthesized by a simple and easy chemical method from ZnCl2. The synthesized nanoparticle was characterized by SEM and EDAX. Antibacterial activity of ZnO was observed against E. coli, Enterobacter sp., Bacillus subtilis, Bacillus cereus, Klebsiella pneumoniae, Staphylococcus aureus, Salmonella typhi, Vibrio cholerae by disc diffusion method. Preliminary Cytotoxicity of nanoparticle was studied by MTT assay against vero cell line. ZnO nanoparticle had shown antibacterial activity against all the tested bacteria and maximum activity was observed against salmonella typhi. ZnO nanoparticles has shown less cytotoxicity effect on vero cell than ZnCl2.
K. Renugadevi*, P. Raji and M. Bavanilatha
Department of Biotechnology, Sathyabama University, Rajiv Gandhi Salai, Chennai- 600 119, Tamil Nadu, India
24 April, 2012
14 May, 2012
21 July, 2012
01 August, 2012