IN-VITRO EVALUATION OF ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACT FROM THE STEM BARK OF ANNONA SENEGALENSIS PERS.Abstract
The antibacterial efficacy of the crude methanolic and aqueous extracts of Annona senegalensis, Pers on clinical isolates of E. coli, Salmonella enteriditis and Shigella dysenteriae were determined using Agar Well Diffusion technique on Mueller Hinton Agar (MHA) plates. At the concentration of between 100 and 200mg/ml, the methanolic extract inhibited the growth of all the organisms with an average zone of inhibition diameter ranging from 30±0.2 to 35±0.36mm for E. coli and S. Enteriditis while for S. dysenteriae, it ranged from 21±0.22 to 26±0.36mm. On the other hand, the aqueous extract did not exhibit activity on any of the test organisms. The Minimum Inhibitory Concentration (MIC) of the methanolic extract on the organisms ranged from 0.39mg/ml on E. coli, 3.17mg/ml on S. enteriditis 25.00mg/ml on S. dysenteriae. The result from this study suggests that the crude extract from the stem bark of A. senegalensis could be used in the treatment of gastroenteritis caused by these organisms.