IN-VITRO EVALUATION OF ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF TYLOPHORA INDICA (BURM F.) MERILLAbstract
Tylophora indica is a perennial climbing plant native to India, found in plains, forests, hills of southern and eastern India. The portions of plant used medicinally are leaves. The Total phenolic content of methanolic leaves extract was estimated by Folin-Ciocalteu assay method, and was found to be 0.160 mg/CE/g (Catechine Equivalent per gram). However, antioxidant activity of methanolic leaves extract of Tylophora indica was determined by DPPH free radical scavenging method. The DPPH radical scavenging activity of methanolic extract of Tylophora indica was found to be highest at 100μl concentration which was 30.74%. Nevertheless, % DPPH scavenging activity of standard ascorbic acid at same concentration was found to be 45.43%. The % DPPH scavenging activity increases with the increasing concentration. The concentration of Tylophora indica needed for 50% inhibition (IC50) was found to be 199.58μg/ml whereas 194.58 μg/ml needed for ascorbic acid.