IN-VITRO FREE-RADICAL SCAVENGING POTENTIAL OF THREE LIVERWORTS OF DARJEELING HIMALAYAAbstract
The present study was aimed to screen the antioxidant activity and phytochemical content of bryophyte species Marchantia paleacea Bertol., Marchantia linearis Lehm. & Lindenb. and Conocephalum conicum (L.) Underw. collected from Darjeeling hills of Eastern Himalaya, India. The free radical scavenging activities were measured in-vitro by DPPH radical, metal chelating, superoxide, ABTS+, reducing power, anti-lipid peroxidation, and nitric oxide scavenging activity. Total phenol, flavonoids, and ortho-dihydric phenol present in the samples were also estimated. Qualitative phytochemical screening was carried out to detect the presence of varied phytochemicals. Thin-layer chromatography (TLC) and TLC bioautography assay were also performed to confirm the presence of different bioactive compounds and its free radical scavenging potential. All the tested bryophyte species showed potential antioxidant activity and the existence of different phytochemicals. The results obtained from this work indicated that all the three bryophyte species analyzed are a potent source for antioxidants and can be pharmaceutically explored in future.
S. Mukhia, P. Mandal *, D. K. Singh, D. Singh and D. Choudhury
Plant Physiology and Pharmacognosy Research Laboratory, Department of Botany, North Bengal University, Raja Rammohunpur, Darjeeling, West Bengal, India.
24 March 2014
25 April 2014
11 July 2014
01 October 2014