IN-VITRO INHIBITION AND DISSOLUTION OF CALCIUM OXALATE STONES BY THE FRACTIONS OF THE ZIZIPHUS LOTUS L. LEAVES

Nowadays, many clinical studies have provided reliable results on the effects of several herbal medicines on many diseases such as kidney problems. The objective of this study is to evaluate in-vitro the antiurolithiatic activity of polyphenols, flavonoids, and alkaloids fractions of the leaves of the species Ziziphus lotus. The quantification of the polyphenol and flavonoid fractions was carried out by spectrophotometric methods; on the other hand, the alkaloid fraction is quantified gravimetrically. The antiurolithiatic activity of the fractions is studied by the use of the turbidimetric and gravimetric techniques. The polyphenol content varies between 1198,696 ± 99,848 and 15270,751 ± 290,419 (μg GAE)/g). While flavonoids range from 97,340 ± 8,437 to 1745,864 ± 8,690 (μgQE/g). On the other hand, the alkaloid fraction represents 1. 26%. The polyphenols fraction showed a higher inhibition 89.80 ± 0.69 than the two other fractions which represent the values 84.17 ± 1.11 and 78.90 ± 1.40, respectively. The polyphenols fraction (AF: 86.66 ± 1.43) and flavonoids (DMF: 80.85 ± 2.03) show an increasing evolution of the capacity to dissolve the precipitate of calcium oxalate compared to the reference solution (Sodium citrate: 75.38 ± 2.03). On the other hand, the alkaloid fraction represents a less significant dissolving power (AlF: 66.81 ± 4.13). The antiurolithiatic activity of Ziziphus lotus leaves is due to the presence of bioactive compounds such as polyphenols, flavonoids, and alkaloids, these results deserve further study to isolate the active substances responsible for the inhibition and dissolution of crystals.