IN-VITRO MUTAGENIC AND CYTOTOXIC ACTIVITY OF NIGELLA SATIVA SEED EXTRACT BY AMES AND MTT ASSAYAbstract
In the modern lifestyle, people are prone to disease. The epidemiology study shows that the lung and colon cancer leading position in both the gender around the world at any group. As based on the traditional systems of medicine, the plants can be used for the discovery of new medicine. The aqueous and ethanol extract of Nigella sativa (N. sativa) seeds were evaluated for their genotoxicity using Ames test and cytotoxicity by MTT assay. Ames assay was conducted using strains of Salmonella typhimurium TA-100, whereas MTT assay was performed on human alveolar basal epithelial cells (A549) and human colon cancer cell line (HCT15). There is no mutagenic activity was obtained when treated with aqueous and ethanol extract of Nigella sativa seeds. But both the extracts were found to be cytotoxic against the tested cancer cell lines. On the basis of the results, it was concluded that aqueous and ethanol extracts of Nigella sativa seeds possess cytotoxic potential but not mutagenic. It is suggested to explore the seeds further to evaluate its anticancer activity in the in-vivo model.
B. Durga *, M. V. D. Prakash and A. Julius
Department of Biochemistry, Prince Shri Venkateshwara Arts and Science College, Chennai, Tamil Nadu, India.
14 November 2019
27 March 2020
29 March 2020
01 November 2020