IN VITRO PROPAGATION FROM INFLORESCENCE EXPLANTS OF ANISOCHILUS CARNOSUS- AN ETHNO-MEDICINAL HERBAbstract
A protocol was devised for callus induction and in vitro micro propagation of Anisochilus carnosus. Leaf, nodal and immature inflorescence explants were cultured for callusing on solid MS medium with best callusing response achieved at higher concentrations of BAP (12mg/l) alone and in combination with 2,4-D (3mg/l) and IAA (5mg/l). Bud regeneration from inflorescence callus giving maximum (9.67±0.58) shoots per bud was achieved at 12mg/l BAP+5mg/l IAA. Elongated shoots were transferred to half strength MS liquid medium for in vitro rhizogenesis, with best rooting achieved in 2mg/l IBA giving maximum number of 13.00±1.00 roots per shoot. The rooted plantlets were hardened by transferring to polycups containing sterile soil and vermicompost in the ratio of 1:1. After 15 days of hardening, established plantlets were transferred to field with maximum survivability.
Nissar Ahmad Reshi*, M.S. Sudarshana and B.P. Nandini
Plant Tissue Culture Laboratory, Department of Studies in Botany, University of Mysore, Mysore-570 006, Karnataka, India
06 October, 2013
19 February, 2014
03 May, 2014
01, June 2014