IN VITRO REGENERATION OF MEDICINAL PLANT EPHEDRA GERARDIANAAbstract
Ephedra gerardiana is an important medicinal plant used as Somalata in Indian Ayurvedic system of medicine and as traditional Chinese medicine since several thousand years. Plant is known to contain valuable secondary metabolite such as ephedrine and pseudo ephedrine. These secondary metabolites are utilized for several medicinal purposes. The present study describes the in vitro regeneration of Ephedra gerardiana using tissue culture technique. Experiments were conducted to study the effect of various combinations of auxins (IBA) and cytokinins (BAP) alone or in different combinations on in vitro culture of nodal segments. Shoot induction and elongation from cultured nodal segments were obtained onto MS medium supplemented with various concentrations of BAP and 15 µM Kinetin. MS+IBA media was utilized for in vitro root induction. Among different media combinations utilized MS+ 5 µM BAP+ 15 µM kinetin was found best for shooting and ¼ MS+ 20 µM IBA was most appropriate for rooting.
I. Rautela, M. Dhiman, M. D. Sharma and P. Misra*
Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, Uttar Pradesh, India.
20 June, 2017
15 December, 2017
06 January, 2018
01 March, 2018